CCR7^loCXCR3^hi sorted from healthy human peripheral blood show effector/effector memory gene expression pattern. (A) The heat-map represents the normalized expression values of CD8⁺CCR7^hiCXCR3^lo (R7^hi) and CD8⁺CCR7^loCXCR3^hi (XR3^hi) cells isolated from 4 individual healthy human donors (D6-D9) by FACS sorting. A statistically significant (P ≤ .01) gene list was obtained from the 2 subsets using a pair-wise t test. A list of differentially regulated genes (≥ 1.7-fold) in 4 donors was generated, and hierarchical clustering of both cell types and entities was performed. (B) Genes with higher expression values in CXCR3^hi or CCR7^hi cells (compared with each other) were used to determine Functional Annotation Clustering of Biological Processes using DAVID. Enrichment score of the cluster is used as an indication for how strongly groups of differentially expressed genes are involved within each functional cluster. (C) Normalized expression values of selected genes are plotted as box-whisker bars (whiskers represent range).