Treatment with DZNep and/or PS induces differentiation of AML cells. (A) Immunoblot analysis of HL-60 and OCI-AML3 cells treated for 48 hours with the indicated concentrations of DZNep and/or PS. The expression levels of β-actin in the lysates served as the loading control. Vertical lines have been inserted to indicate repositioned gel lanes. (B) HL-60 and OCI-AML3 cells were treated with the indicated concentrations of DZNep and/or PS for 48 hours. After this, cells were washed and stained with CD11b antibody, and the percentages of CD11b⁺ cells were determined by flow cytometry. (C) HL-60 and OCI-AML3 cells were treated with the indicated concentrations of DZNep and/or PS for 72 hours. After treatment, the cells were cytospun onto glass slides, Wright stained, and observed with a microscope to assess cellular morphology.