IFN-β influences pDC migration during inflammation and under homeostatic conditions. (A) C57Bl/6 mice or IFN-β^−/− mice were either mock-infected (PBS) or infected with influenza virus intranasally. After 18 hours, MLNs were collected, and B cells, T cells, and pDCs were analyzed by flow cytometry. Numbers of lymphocytes or pDCs in the MLNs of influenza-infected mice were compared with the number in MLNs of uninfected mice to obtain a LN expansion ratio. The data are representative of 2 independent experiments. (B) IFN-β influences pDC migration under homeostatic conditions. LNs (left column) and spleens (right column) were harvested from WT C57BL/6 mice or IFN-β^−/− mice and B cells, T cells. and pDCs in LNs and spleen of WT versus IFN-β^−/− mice were assessed by flow cytometry. (C) CD69 levels on B cells, T cells, and pDCs from WT versus IFN-β^−/− mice were evaluated by flow cytometry. Data are from 3 independent experiments. *P < .05, **P < .005.