Administration of recombinant IFN-β restores balance of pDCs and lymphocytes in the LNs of IFN-β−/− mice. Naive (A) and influenza-infected (B) WT C57Bl/6 mice or IFN-β−/− mice were treated with 105 U recombinant IFN-β by intraperitoneal injection. After 18 hours, blood and LNs were harvested, and ratios of lymphocyte or pDC numbers in LNs/blood were obtained by flow cytometry. A total of n = 5-7 mice per group per experiment was examined, and the experiment was performed twice. *P < .05, **P < .005, ***P < .0005. (C) CD69 levels on pDCs from IFN-β−/− mice were determined 18 hours after influenza infection, with and without the addition of recombinant IFN-β. A representative example of CD69 staining on pDC is shown from a total of 5 mice. Shaded histogram indicates pDCs from uninfected mice. Broken line histogram, pDCs from influenza infected mice. Solid line histogram, pDCs from influenza-infected mice treated with recombinant IFN-β.
