Thrombin induces phosphorylation of ERK1/2 and p38. (A-B) MDMs were incubated with α-thrombin (1 U/mL) for indicated time periods or preincubated for 30 minutes with the ERK1/2 inhibitor PD98059 (30 μM) or with the p38 inhibitor SB202190 (30 μM) and thereafter treated with α-thrombin (1 U/mL) for 25 minutes. Phosphorylation of the respective kinases was determined as described in “Determination of the phosphorylation of MAPKs.” Values are given in mean density and represent mean values ± SD of 3 independent determinations. Experiments were performed 2 times with MDMs obtained from 2 different donors with similar results. A representative experiment is shown. (C) MDMs were preincubated for 30 minutes with the ERK1/2 inhibitor PD98059 (30 μM) or with the p38 inhibitor SB202190 (30 μM), and thereafter treated with α-thrombin (1 U/mL) for 24 hours. Conditioned media of such treated cells were collected and OSM antigen was determined as described in “Quantification of OSM.” Values are given in nanograms per milliliter and represent mean values ± SD of 3 independent determinations. Experiments were performed 2 times with MDMs obtained from 2 different donors with similar results. A representative experiment is shown. *P < .001 (ERK1); §P < .001 (ERK2); #P < .001 (p38).