Dynamic epigenetic gene regulations in IL-4–induced M2-macrophages. (A) BMDMs were incubated with or without IL-4 for 2 days. ChIP assay was performed on the promoter regions of Chi3l3, Retnla, and Arg1 using indicated antibodies. (B-C) BMDMs were incubated with IL-4 for 2 days and then were cultured with (IL-4 continuous group) or without (IL-4 medium group) IL-4 for an additional 2 days. ChIP assay was performed on the promoter regions of M2 marker genes using antibodies for H3K4me3 (B) and H3K27me3 (C). Untreated cells were used as a baseline control (medium-medium group). Data are representative of 3 to 5 independent experiments and are expressed as mean ± SEM. *P < .05; **P < .01.