STAT6-dependent Jmjd3 induction in IL-4–induced M2-macrophages. (A) Immunoblotting for STAT6 in BMDMs from WT and Stat6^−/− mice. Gapdh was used as a loading control. (B-C) The expression of Jmjd3 (B), Chi3l3, Retnla, Arg1, and Nos2 (C) in WT and Stat6^−/− BMDMs was measured by quantitative RT-PCR after the indicated treatments. The data shown are “fold induction” relative to that in untreated WT cells. (D) Anti-H3K27me3 ChIP assay was performed on the promoter regions of M2 marker genes in WT and Stat6^−/− BMDMs after IL-4 stimulation. Data are representative of 3 independent experiments and are expressed as mean ± SEM. *P < .05, **P < .01, compared with WT mice group (B-C) or unstimulated (medium alone) group (D).