Translocation of YFP-p40^phox or YFP-p40^phoxR105Q during phagocytosis of SOZ. Confocal videomicroscopy was used to monitor phagosome translocation of YFP-p40^phox and YFP-p40^phoxR105Q expressed in PLB-p40 KD granulocytes. (A) Time-lapse images. Times shown are referenced to time of phagosome sealing. Arrow indicates a monitored SOZ phagosome; N, nucleus. (B) Phagosomes that showed accumulation of wild-type or mutant YFP-tagged p40^phox during phagosome cup formation were monitored for continued presence of the YFP-tagged proteins for 220 seconds after sealing. Solid line indicates YFP-p40^phoxWT; dashed line, YFP-p40^phoxR105Q. Data for YFP-p40^phoxR105Q and YFP-p40^phoxWT were collected from 10 and 5 phagosomes, respectively, as filmed in 3 independent experiments.