GPR65 is a critical regulator of BAL eosinophilia and viability in murine asthma model. Sensitized GPR65^+/+ and GPR65^−/− were challenged with intranasal OVA or saline. The differential cell counts were performed by staining cytocentrifuge preparations of the BALF (A). n = 4-6 mice per group. Flow cytometry–based viability analysis of eosinophils and mononuclear cells in representative in vivo experiment using annexin V/7AAD (B) and anti-active caspase-3 (C). n = 4-6 mice per group. Results are shown as the mean ± SD. Representative experiments (of 3-5) are shown.