![Figure 5. Binding between ADAMTS13 and VWF fragments under flow conditions determined by SPR. Different concentrations (0-3.5 μM) of VWF fragments (VWFA3CK, VWFD4CK, VWFD4, and VWFB1CK) were injected at a flow rate of 30 μL/min over flow cell 1 and flow cell 2 of a sensor chip, on which BSA (∼ 2000-3000 response units [RU]) and ADAMTS13 (∼ 4000-8000 RU) were immobilized, respectively. The curves representing the binding of ADAMTS13 to VWFA3CK, VWFD4CK, VWFD4, and VWFB1CK are shown in panels A, C, E, and G, respectively. The curves are the subtraction between the signals registered in flow cell 2 and in flow cell 1. The association and dissociation phases of the derived sensograms were fitted to 1:1 Langmuir model, giving values for ka, kd, and KD. The plotting of the RUmax at each substrate concentration of VWFA3CK, VWFD4CK, VWFD4, and VWFB1CK is shown in panels B, D, F, and G, respectively. These curves were fitted to the one-binding site model, giving values for KD constants.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/13/10.1182_blood-2009-05-224915/4/zh89990941300005.jpeg?Expires=1735562935&Signature=izY9sHd26COmevnVZXGWQYXJx6wTHjBp1vXHY9-NvKua2UZisPllZQi8dUY3izjyM0DKgaRS77dKV8jJvVE8Uu6o8LeHyN2f3ZRk0-irpbG800ggCuayDr0B3VsjZhjEaMafVGfUm3ybxC2L5ozbSai2dtaF0~dM5UQ6Gu~CPCcMLYPcac-stCjAwvA03ISnnNuZbxlSM2GKlrkkN55ukzf6WxsqD49xaXw~wjrE77wiYQetcT-qMN3bPrn91CEYj9RCXaGIZYcSQQrAQBXc4m9YSv5wQ43vjuJ9koeiherC84wl4EYC6ENhGbEzPTJB00rBanTiVOCryn76MVld7Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Binding between ADAMTS13 and VWF fragments under flow conditions determined by SPR. Different concentrations (0-3.5 μM) of VWF fragments (VWFA3CK, VWFD4CK, VWFD4, and VWFB1CK) were injected at a flow rate of 30 μL/min over flow cell 1 and flow cell 2 of a sensor chip, on which BSA (∼ 2000-3000 response units [RU]) and ADAMTS13 (∼ 4000-8000 RU) were immobilized, respectively. The curves representing the binding of ADAMTS13 to VWFA3CK, VWFD4CK, VWFD4, and VWFB1CK are shown in panels A, C, E, and G, respectively. The curves are the subtraction between the signals registered in flow cell 2 and in flow cell 1. The association and dissociation phases of the derived sensograms were fitted to 1:1 Langmuir model, giving values for ka, kd, and KD. The plotting of the RUmax at each substrate concentration of VWFA3CK, VWFD4CK, VWFD4, and VWFB1CK is shown in panels B, D, F, and G, respectively. These curves were fitted to the one-binding site model, giving values for KD constants.
