BMMCs, Tregs, and Th17 cells populate inflamed airways. AHR was induced in OTII mice by intranasal OVA injection (A). BAL cells were collected 24 hours after the last inhalation. (B) Representative plots showing surface FcϵRI expression and IL-6 intracellular content in gated CD45+ c-kithigh (middle panel), representing MCs among harvested BAL cells, and in gated CD3+ lymphocytes (right panel). Among gated CD4+ cells, IL-17 versus Foxp3 expression is depicted (C). Histogram shows intracellular TGF-β expression in gated CD4+Foxp3+ Tregs (solid line) compared with isotype control (filled area). The shown data are representative from 3 experiments, each including at least 5 mice.