Eltrombopag does not increase relative percentages of leukemic blasts. (A) Relative numbers of CD33-expressing myeloid cells in cultures of MDS/AML patient-derived BM-MNC in the presence of Eltrombopag. BM-MNC of MDS/AML patients were seeded in liquid cultures containing BSA, insulin, and transferrin, supplemented with hrIL-3, hrIL-6, hrSCF, and hrFLT3L. Cells were incubated with either 0 or 3 μg/mL Eltrombopag or 100 ng/mL hrTPO for 12 days. CD33⁺ cells were assessed by FACS analysis. Results from 7 individual patients are shown. (B) Relative abundance of leukemic blasts and CD33⁺ cells in cultures of MDS/AML patient-derived BM-MNC in presence of Eltrombopag. BM-MNC of MDS/AML patients were grown in cytokine-supplemented medium and incubated with either 0 or 3 μg/mL Eltrombopag for 5 days. Relative blast counts were assessed from cytospin preparations, and CD33⁺ cells were assessed by FACS analysis. Results from 4 individual patients are shown. (C) Relative abundance of leukemic blasts after plating of MDS BM-MNC in methylcellulose. BM-MNC were cultured in cytokine-supplemented methylcellulose for 12 days and then replated, in the presence or absence of Eltrombopag or 100 ng/mL hrTPO. Cytospins were prepared and stained with Wright-Giemsa after the primary plating as well as the replating. Pictures of a representative MDS patient are shown. Blasts are indicated by arrows.