Gene expression profile in purified T-cell subset from GT-treated patients. Heat maps representing the unsupervised Affymetrix gene expression analysis of PB CD4⁺ (A) and CD8⁺ (B) T cells isolated from 3 GT-treated patients (patient 1, +30 months; patient 3, +26 months; patient 4, +10 months) and 4 age-matched healthy controls (C). Expression levels are represented by a color key in which bright red represents the highest levels (up-regulated genes) and bright blue represents the lowest levels (down-regulated genes); less saturated shades represent intermediate levels of expression. Heat maps resulting from supervised hierarchical clustering of CD4⁺ (C) and CD8⁺ (D) T-cell samples according to the type of treatment (ie, GT vs normal controls). P value cutoff = .05; multiple testing correction, none. This restriction tested 9364 genes. Approximately 4682 genes would be expected to pass the restriction by chance.