Analysis of TCR diversity and hematopoietic origin of T-cell clones. (A) Immune repertoire of T-cell clones isolated from the PB of patient 2 and patient 3. Each bar represents the percentage of clones displaying the specific TCR Vβ chain rearrangement, as analyzed by flow cytometry. (B) Analysis of Vβ repertoire in 3 clones from patient 3 sharing a common RIS. (C) Quantification of specific RIS-containing clones (ID-04 and ID-13 from patient 2 identified in 5 and 2 independent T-cell clones; ID-16 and ID-30 from patient 3 identified in 3 and one T-cell clone, respectively) within the total T-cell population. The frequency of insertions was measured by sequence-specific real-time PCR in CD3⁺ T cells purified from PB at different time points (months) during the follow-up after GT. Rectangle represents the time point at which the T-cell cloning was performed. (D) T-cell clones from patient 3 share RIS with distinct hematopoietic cell subsets. Integration analysis was performed, by random cloning and sequence-specific PCR, on cells purified ex vivo from PB or bone marrow (BM) at different time points (months [mo]) during the follow-up.