American Society of Hematology

Figure 2

$Figure 2. MMc in hematopoetic versus parenchymal cell lineages. (A) Cells from spleen and bone marrow of 6 NIMAd-exposed mice (standard breeding) were separated by expression of CD4, CD8, CD11b, and CD11c using MACS beads, and DNA was extracted from separated populations and tested for H2Dd MMc. The figure shows levels of MMc (GEq/105 sorted cells) in the sorted cell populations. Individual mice are represented by symbols as shown. (B) Cells of hematopoietic lineages (lineage+) and nonhematopoietic lineages (lineage−) were sorted from single-cell suspensions of heart tissue pooled from 8 NIMAd-exposed mice. Lineage+ cells were sorted with a lineage cell depletion kit containing a cocktail of monoclonal antibodies against CD5, CD45R (B220), CD11b, Gr-1 (Ly-6G/C), 7-4, and Ter-119. Maternal cells were detected using H2Dd qPCR from the sorted fractions. The dots represent replicate values of analysis of the pooled lineage+ or lineage− cell DNA sample.$

MMc in hematopoetic versus parenchymal cell lineages. (A) Cells from spleen and bone marrow of 6 NIMA^d-exposed mice (standard breeding) were separated by expression of CD4, CD8, CD11b, and CD11c using MACS beads, and DNA was extracted from separated populations and tested for H2D^d MMc. The figure shows levels of MMc (GEq/10⁵ sorted cells) in the sorted cell populations. Individual mice are represented by symbols as shown. (B) Cells of hematopoietic lineages (lineage⁺) and nonhematopoietic lineages (lineage⁻) were sorted from single-cell suspensions of heart tissue pooled from 8 NIMA^d-exposed mice. Lineage⁺ cells were sorted with a lineage cell depletion kit containing a cocktail of monoclonal antibodies against CD5, CD45R (B220), CD11b, Gr-1 (Ly-6G/C), 7-4, and Ter-119. Maternal cells were detected using H2D^d qPCR from the sorted fractions. The dots represent replicate values of analysis of the pooled lineage⁺ or lineage⁻ cell DNA sample.

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