Cells of NIMA^d-exposed mice express low levels of maternal MHC class I antigen. Cells from thymus, spleen, pooled LNs, bone marrow, and blood of NIMA^d-exposed and NIPA^d control mice were stained with anti-H2K^d antibody and analyzed by flow cytometry. A minimum of 10⁶ events were collected for each sample. All cells in the live gate (propidium iodide–excluding) were included in the analysis. (A) An example of flow histograms of H2K^d expression on peripheral blood mononuclear cells (PBMCs) from a NIMA^d-exposed, NIPA^d control, B6 negative control, and BDF₁ positive control mouse. (B) Summary data of percentage of H2K^d-dim cells in various lymphoid organs of NIMA^d-exposed mice (▵) and NIPA^d control mice (●). B6 background values have been subtracted. The horizontal line indicates the lower limit of sensitivity by flow cytometry. (C) Example of levels of maternal H2K^d-dim offspring cells in blood at 4 different time points after weaning. (D) Comparison of peak percentages of maternal antigen-dim offspring cells (from 4 different time points tested) among 3 groups of offspring. The error bars represent SEM.