Regenerative potential of ATL-like lymphoma in 10² CSCs. ATL-like lymphoma-regenerative activity was assessed by the transplantation of 10² CSCs, the non-CSC fraction, and SLCs. (A) ATL-like lymphoma was regenerated by the transplantation of 10² CSCs into the NOD/SCID mouse at 60 days. (B) Marked splenomegaly was observed in the NOD/SCID recipient mice. (C) Ascites was also observed in the NOD/SCID recipient mice. (D) Total spleen weight was significantly increased only in the CSC-transplanted NOD/SCID mouse. (E) SP analysis of ATL-like lymphoma cells generated by the transplantation of 10² CSCs. (Left panel) The SP fraction (total = 0.066%) was present after CSC transplantation. (Right panel) The SP fraction was lost in the dot plot with treatment by 100 μM verapamil. (F) Surface marker analysis in the lymphoma cells regenerated by the 10² CSC transplantation. The CSC candidate cells (CD38⁻/CD71⁻/CD117⁺) were also regenerated after transplantation of 10² CSCs.