Histologic and immunohistochemical analyses of spleen after transplantation. At 60 days, lymphoma was regenerated after the transplantation of 10⁵ SLCs and 10² CSCs. No lymphoma was observed after the transplantation of 10² non-CSCs. (A) PAS-hematoxylin staining in the 10⁵ SLCs recipient spleen. (Top panel) Low magnification. (Bottom panel) High magnification. Strong PAS⁺-stained cells were observed (). (B) PAS-hematoxylin staining in the 1 × 10² CSCs recipient spleen. (C) PAS-hematoxylin staining in the 1 × 10² non-CSCs recipient spleen. No strong PAS⁺ staining cells were evident. (D-I) Immunohistochemistry of CD44 and CD117, shown in the 10⁵ SLC recipient spleen (D,G), in the 10² CSC recipient spleen (E,H), and in the 1 × 10² SLC recipient spleen (F,I). CD44 and CD117 expression is detected in the lymphoma in the spleen after 1 × 10⁵ SLC and 1 × 10² CSC transplantation. (J) Hematoxylin and eosin (H&E) staining of the normal NOD/SCID mouse BM. Various types of blood cells, including megakaryocytes and erythroid cells, were evident. (K) Hematoxylin and eosin staining of lymphoma reconstituted in the NOD/SCID mouse BM. The BM tissue was uniformly filled with ATL-like lymphomatous cells. (L) Infiltration of lymphomatous cells was also observed in the liver. (M) Infiltration of lymphomatous cells in lung. (N) Infiltration of lymphomatous cells in lymph nodes. (O) Infiltration of lymphomatous cells was not observed in the epidermal tissues. *Lymphomatous cells. (Closed scale bar, 50 μm; open scale bar, 20 μm.)