Flow cytometric analysis of lymphomatous cells in the NOD/SCID BM. NOD/SCID repopulating lymphomatous cells were isolated from BM. (A) Triple staining analysis with CD38, CD71, and CD117 in the BM SLCs. As was observed in spleen, the CSC candidates were also observed in the BM. (B) Histologic analysis of ATL-like lymphomatous cells in the NOD/SCID BM. In the normal NOD/SCID BM, CD3⁺ cells were a rare population (). (C) In the reconstituted NOD/SCID mouse BM, CD3⁺ cells were readily identified (arrow). (D) CD117⁺ cells as a surrogate CSC marker could be detected in the osteoblastic niche of the trabecular bone (arrow). (E) In the medullary region, CD117⁺ cells (CSCs) were also detected in the vascular niche (). T.b. indicates endosteal region in the trabecular bone; V, vascular zone; TP+, transplantation; and NT, no transplantation. Scale bar represents 20 μm.