A constitutively activated AKT inhibits macrophage differentiation. Monocytes were transfected with an empty vector (pcDNA) or the vector encoding a constitutively active mutant of AKT (Myr-AKT). Cells were then treated for 3 days with CSF-1 before qPCR analysis of indicated gene expression (mean ± SD of triplicates; A), morphologic examination of cells (B), and immunoblot analysis of indicated proteins (C). Molecular weight (MW) stated as kilodaltons. *Cleavage fragment. pp70 S6K is used as a marker of AKT activity.