Figure 4
![Figure 4. Warfarin prevents the initiation of an immune response to FVIII. FVIII−/−/BALB/c mice were pretreated with warfarin (n = 6) or PBS (n = 7) before the first and third of 5 intraperitoneal injections of 1 μg FVIII. This experiment was repeated twice, and representative data are shown. Seven days after the last injection, (A) serum samples were analyzed for antibody response (582 ± 232 [FVIII] vs 101 ± 24 μg/mL [FVIII + warfarin]; *P = .042) and (B) splenic T cells from the mice treated with FVIII or FVIII + warfarin were assayed for the response to FVIII (**P = .002). These data indicate that other coagulation factors besides FVIII are probably necessary for initiation of the immune response.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/21/10.1182_blood-2008-10-186452/4/zh89990944920004.jpeg?Expires=1736059077&Signature=nO6pge4WwfF4GfT912nBgAQeXPuK7~nIWZbsQe9C9S5xYJ8oSO4lrl5b6xF~4jlCD6UcsL39FfOh64RxImuTWDmE1zWp7OW614LV2j6bJAhuDXkRtFFAodbDlJi6iFoWScjEe0WgJk1XdpsJnbxB0zhzlhhDZt28wmflf-ufwbBLKZOflJpIuJ7pgQZJ~mqCh2SH5xyvuAuylFTkkU1sVcTATXy5aEvYtYUFN-Q9HEMMXHvBt1CY3My62jxxW5coQ3J6-c1zJtZM~I8j-07st3r1KuVtbxjM1MP8IxV79MVvigAbpP4ihm0p2bvOkNJw1pmI81ry-VOzHM68gZ1GAQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Warfarin prevents the initiation of an immune response to FVIII. FVIII−/−/BALB/c mice were pretreated with warfarin (n = 6) or PBS (n = 7) before the first and third of 5 intraperitoneal injections of 1 μg FVIII. This experiment was repeated twice, and representative data are shown. Seven days after the last injection, (A) serum samples were analyzed for antibody response (582 ± 232 [FVIII] vs 101 ± 24 μg/mL [FVIII + warfarin]; *P = .042) and (B) splenic T cells from the mice treated with FVIII or FVIII + warfarin were assayed for the response to FVIII (**P = .002). These data indicate that other coagulation factors besides FVIII are probably necessary for initiation of the immune response.
