Figure 5
![Figure 5. Hirudin inactivates thrombin and reduces the immunogenicity of FVIII. FVIII−/−/C57BL/6 mice received 5 intraperitoneal injections of 1 μg FVIII (n = 5) only or 1 μg FVIII with 200 μg hirudin (n = 5). This experiment was repeated twice, and representative results are reported here. Seven days after the last injection, (A) serum samples were analyzed for antibody response (515 ± 168 [FVIII] vs 134 ± 16 [FVIII + hirudin] μg/mL; *P = .02) and (B) splenic T cells were assayed for the response to FVIII (*P < .002). These data indicate that thrombin is necessary for initiation of the immune response to FVIII.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/21/10.1182_blood-2008-10-186452/4/zh89990944920005.jpeg?Expires=1736058838&Signature=whbKl3AVpEYqL3tRgoO7gZnD69YPNE4f1WzgNFNyOIjDx104cN5cYPFf72RpKMlgZYqXX-K9~mJSJso3UYb1JisE1fsCw4NpFppTbAWFSQDTxACKCoG1b4ZNjVzJHRNXwQzFGaAWAsJu00AI7GyLRfcK57Rv-3SQf-6urD3YbXU3e~2b8yHCnkQXcoE6Y74qSI1rm9E3PGK9fvO70e8OZSjdydYC7jmDeb4NnnTmw93GK4hr7a5gM3tCINm1om1JGuw3MwftmudhNMpqFsx9Sq8TM7V4xEO8LG8VY3771RBAvpMWRjZOHCIoNfRFkZI~RyPoRK9tHNTnKhitMM8KpA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Hirudin inactivates thrombin and reduces the immunogenicity of FVIII. FVIII−/−/C57BL/6 mice received 5 intraperitoneal injections of 1 μg FVIII (n = 5) only or 1 μg FVIII with 200 μg hirudin (n = 5). This experiment was repeated twice, and representative results are reported here. Seven days after the last injection, (A) serum samples were analyzed for antibody response (515 ± 168 [FVIII] vs 134 ± 16 [FVIII + hirudin] μg/mL; *P = .02) and (B) splenic T cells were assayed for the response to FVIII (*P < .002). These data indicate that thrombin is necessary for initiation of the immune response to FVIII.
