American Society of Hematology

Figure 5

$Figure 5. Hsp90 knockdown by siRNA reduces proliferation in mixed lymphocyte reactions. (A) Flow cytometric analysis of CD3+ T cells electroporated with NGFR-specific mRNA to estimate transfection efficiency in primary human lymphocytes. Transfection efficiency was 59% for CD8+ cells and 55% for CD4+ cells. (B) Genetic knockdown of Hsp90β mRNA with siRNA in resting or activated CD3+ T cells, as determined by real-time PCR. (C) CFSE dilution after 4 days in MLRs of PBMC electroporated either with control siRNA or Hsp90β-specific siRNA. (D) Summary of depletion efficacy in 3 experiments: proliferative fraction of MLRs transfected with control siRNA or Hsp90-specific siRNA. Displayed P values were obtained using a 2-tailed paired t test.$

Hsp90 knockdown by siRNA reduces proliferation in mixed lymphocyte reactions. (A) Flow cytometric analysis of CD3⁺ T cells electroporated with NGFR-specific mRNA to estimate transfection efficiency in primary human lymphocytes. Transfection efficiency was 59% for CD8⁺ cells and 55% for CD4⁺ cells. (B) Genetic knockdown of Hsp90β mRNA with siRNA in resting or activated CD3⁺ T cells, as determined by real-time PCR. (C) CFSE dilution after 4 days in MLRs of PBMC electroporated either with control siRNA or Hsp90β-specific siRNA. (D) Summary of depletion efficacy in 3 experiments: proliferative fraction of MLRs transfected with control siRNA or Hsp90-specific siRNA. Displayed P values were obtained using a 2-tailed paired t test.

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