AML progression results in increased Tregs present at the primary sites of AML metastases. B6 mice were injected intravenously with 106 C1498FFDsR cells and killed 6, 14, or 25 days after tumor injection. Flow cytometric analysis was performed on liver leukocytes and splenocytes. (A) Increased percentage of DsR+ tumor cells was found in the livers and spleens of mice. (B-C) An increased percentage of Foxp3+ Tregs in the CD4+ T-cell population was found in the livers of mice, shown in flow dot plot (B) and bar graph (C, P < .05). (D-E) An increased percentage of Foxp3+ Tregs in the CD4+ T-cell population was found in the spleens of mice, shown in flow dot plot (D) and bar graph (E, P < .05). (F) An increased number of tumor cells was correlated with an increased number of Treg cells in the livers and spleens of mice (P < .05). (G) An increased percentage of Tregs was found in the LNs (P < .05) and blood (P < .05) but not in the BM. Results from 1 of 3 representative experiments are shown. Error bars represent SD.
