Combined IL-2DT treatment restored the proliferation and number of CTLs at sites of AML metastases. Naive or tumor-bearing mice were treated with IL-2DT or control DT (1 μg/dose) 4 and 13 days after tumor injection. Congenic B6-Ly5.2 (CD45.1+) CTLs (30 × 106/dose) were then injected intravenously 14 days after tumor injection and mice were fed with BrdU water to track proliferation. Eighteen days after tumor injection, 4 mice per group were killed. Flow cytometric analysis was done with liver leukocytes and splenocytes. IL-2DT treatment significantly augmented the percentage of BrdU+ CTLs in the spleens compared with control DT treatment. IL-2DT treatment significantly increased the percentage (A) and number (B) of CTLs found in the livers and spleens compared with control DT treatment. Representative data from 1 of 2 similar experiments are shown. Error bars represent SD.
