Protein tyrosine phosphorylation in CRP-stimulated platelets. WT, WASp KO, WIP KO^low, and WIP KO^high platelets were activated with 0.3 or 3 μg/mL CRP for 2 minutes at 37°C as indicated. Platelet lysates and anti–PLC-γ2 immunoprecipitates were subjected to SDS-PAGE and probed with the anti-phosphotyrosine antibody 4G10. The membrane was stripped and probed with anti–PLC-γ2 antibody as loading control. Results are percentage of PLC-γ2 tyrosine phosphorylation compared with WT platelets stimulated with 3 μg/mL CRP and are representative of 3 independent experiments.