Lenalidomide repairs FL T-cell immunologic synapse dysfunction with autologous tumor cells. (A) Autologous T-cell conjugates (with or without sAg-pulsed FL cells stained blue) from untreated (UT) or lenalidomide (Len)–treated (0.5 μM for 24 hours) T and FL cells were scored for accumulation (polarization) of F-actin (red) at the immune synapse. As controls, autologous age-matched healthy T and B cells were used. Data are the mean ± SD from 6 independent experiments with at least 50 conjugates analyzed per experiment. Lenalidomide treatment also enhanced polarization of tyrosine phosphorylated protein (P-Tyr; stained green) to the FL T-cell synapse site. Statistical differences between experimental groups were evaluated by 2-tailed Student t test. P < .05 was considered statistically significant. Nonsignificant findings are denoted by ns. (B) Percentage of FL TIL conjugate efficiency with autologous FL cells (± sAg) before (untreated [UT]) and after lenalidomide (Len) was scored by visual counting using a confocal microscope. Each dataset is the mean ± SD from 6 independent experiments with at least 50 random T cells analyzed per experiment.