Loss of FOG-1 expands myelopoiesis at the expense of erythropoiesis. Lateral views at 24 hpf of Tg(GATA-1:eGFP) embryos uninjected (A,C) or injected with FOG-1MO (B,D). White arrows indicate eGFP expression in the myeloid precursor cells (m); square bracket, ICM expression in insets (C-D). Gated erythroid (red circle) and myeloid cells (blue circle) are shown using forward (FSC) and side scatter (SSC) flow cytometry (E-F). The eGFP⁺ cells were purified by fluorescence-activated flow cytometry (G-H). Histologic analysis of sorted eGFP⁺ cells from FOG-1 morphants shows myelomonocytic (H asterisks) and dyserythropoietic morphology (H arrows); in comparison, the sorted cells from control embryos are predominantly erythroblasts (G). A representative from 3 independent experiments is shown. Populations of cells within the gate are enumerated as mean percentages of total cells ± SD, showing statistically significant differences (*P < .05; E-F).