P aeruginosa's LPS causes defective myeloid differentiation. C57BL/6J mice were injected intraperitoneally with P aeruginosa's LPS (0.8 mg/kg) or PBS. BM was harvested at 24 hours from injection, and Lin⁻ cells were sorted by FACS and grown in vitro in prodifferentiative conditions in the presence of SCF, IL-3, and G-CSF. Cells were harvested at the indicated time points and analyzed for expression of differentiation markers by flow cytometry. (A) Dot blots shows expression of c-Kit and of a combination of myeloid differentiation markers (Gr1 + Mac1 + F4/80) at day 1 and day 3 of culture in a representative experiment. (B) Line graph represents acquisition of the myeloid markers (Gr1, Mac1, F4/80) over time. Values represent average percentages of 3 samples from a representative experiment of 3 independent experiments. Error bars indicate SD. Day 3 *P < .001. (C) Line graph shows kinetic of LSK cells in culture in 3 independent experiments. Values represent average percentage of LSK cells (n = 7).