Hif-2α deletion affects lung endothelial cell adhesion. Reduced adhesion of independently derived KO EC lines to Matrigel- (A) or fibronectin- (B) coated plates, under hypoxic conditions, compared with related control EC lines. MS1, control murine pancreatic endothelial cell line. Cells in 6 random high power fields (HPF) were counted, and combined results from 3 separate experiments are shown. (C-D) Acute HIF-2α deletion in lung endothelial cells from Hif-2α^2L/1L, Ubc-CreER^T2 mice. PCR (C) and Western blot (D) analysis demonstrating efficient deletion of HIF-2α after tamoxifen treatment. Proteins were extracted from cells after exposure to either 21% (N) or 0.5% (H) O₂ for 6 hours. (E-F) Reduced adhesion of ECs with acute HIF-2α deletion to either Matrigel- (E) or fibronectin- (F) coated plates, under hypoxic conditions, compared with related control EC lines. *P < .05; **P < .01.