ZKS cells support hematopoiesis; morphology and gene expression data. (A) Representative hematopoietic cell types recovered from in vitro culture on ZKS stroma. May-Grünwald/Giemsa stained cells were photographed at 1000×. After photographing, cells were cut and pasted from multiple fields to create composite image. (B) Gene expression analysis of cells by RT-PCR recovered from ZKS cultures for the panleukocytic transcript cd45, the erythroid-specific transcript gata1, myeloid-specific transcripts pu.1 and mpx, and the lymphoid-specific transcripts pax5, igM, gata3, lck, and rag2. Gene names listed at left, days in culture on ZKS stroma at top. Whole kidney used as positive controls, dH2O as negative controls. (C) Percentages of lineages in recovered cell populations over 11 days of culture. Percent population listed at left, days in culture on ZKS stroma at bottom. Green indicates myeloid; purple, precursor; blue, lymphoid; and red, erythroid.
