Phenotypically the 2-oncogene (MN1+ND13) and the 1-oncogene (MN1) leukemia model produce similar leukemias. (A) Survival analysis of mice that received a transplant of MN1-transduced (n = 18), MN1+ND13-transduced (n = 13), or control vector-transduced (n = 7) bone marrow cells. (B) Spleen weight, (C) WBC, and (D) RBC counts of moribund leukemic MN1 (n = 10) and MN1+ND13 (n = 5) mice, and of normal control mice (n = 3; mean ± SD). (E) Immunophenotype of leukemic bone marrow cells from moribund MN1 (n = 9) and MN1+ND13 (n = 7) mice gated on transgene-expressing cells (mean ± SD). (F) Representative Wright-Giemsa–stained cytospin preparation of bone marrow cells from leukemic mice, and hematoxylin and eosin–stained tissue sections from spleen and liver of leukemic mice (scale bars represent 10 μm). (G) Survival analysis of mice that received a transplant of MN1+ND13-expressing cells (primary, n = 13) and of leukemic MN1+ND13-expressing leukemic bone marrow (secondary, n = 9).