Contribution of p300-reconstituted cells to hematopoietic cell populations in chimeric mice. (A) Typical flow cytometry gating of lineage⁻ cells for c-Kit and Sca1 expression identifies c-Kit⁺, Lineage⁻, Sca1⁺ (KLS) compartment. Gating of KLS cells determines the percentage that is Ly5.1⁺ (wt blastocyst-derived) versus Ly5.2⁺ (mutant p300 ESC-derived). (B) The contribution of p300-reconstituted (p300 R/−) ESC-derived cells to mouse coat color is compared with their contribution to the KLS compartment. Each dot represents 1 mouse. Simple linear regression with a zero y-intercept creates a best-fit line of the data (slope = 1.017 ± 0.143 SE). (C) Relative contribution of Ly5.2⁺ cells to KLS, CMP, and CLP compartments. Bars indicate the slope of each best fit line graph ± SE as depicted in panel B. (D) Relative contribution of Ly5.2⁺ cells to PB Gr1⁺ cells, B220⁺ (B cells), and Ly1⁺ (T cells) populations. Bars indicate the slope of each best fit line graph ± SE. Dashed line at 1 represents the expected level of hematopoietic contribution if no deficiencies exist (ie, when hematopoietic contribution is equivalent to coat color contribution). Unpaired t tests were used to compare hematopoietic contribution for each genotype with that of wt p300^R/−. Samples that differed significantly from p300^R/− populations are indicated with *P < .05; @P < .01, #P < .001. Number of mice used in each analysis is shown in supplemental Table 3.