Serial replating assay shows that HAT^ptmut KLS cells have greater proliferative potential than wt KLS cells. (A) The number of colonies produced by serially replating wt and HAT^ptmut KLS cells directly in methylcellulose after 1 to 5 weeks in culture. Bars represent the average number of colonies on duplicate dishes for sorted KLS cells from 6 wt mice and 5 HAT^ptmut chimeric mice. (B) The number of weeks that cultures generated colonies (≥ 1 colony per 1000 cells plated) is shown. Dots represent data from individual mice and the horizontal lines represent the average for each set of mice. P values were calculated using the Student t test.