GATA-1 induction mimics VDR-signal inhibition in reestablishing DC development in the presence of 1.25-VD3. CD34⁺ cells were transduced with GATA-1-IRES-GFP, RXRαΔ-IRES-GFP, or empty vector control. Forty-eight hours after transduction, cells were plated in cultures supplemented with LC-promoting cytokines in the presence of 62.5 nmol/L 1.25-VD3 for 7 days. GFP^neg, GFP^lo, and GFP^hi cells were separately gated and were analyzed for CD1a and CD11b expression. Bar diagrams represent mean percentages (± SEM) of CD1a⁺ CD11b⁺ cells in gene-transduced populations as indicated. Each number in a quadrant represents the percentage of cells in the quadrant. (A) GATA-1 versus control vector, n = 5; (B) RXRαΔ versus control vector, n = 3. Western blot shows GATA-1 and actin expression of GFP^lo versus GFP^hi GATA-1-IRES-GFP–transduced cells sorted by FACS. Data are representative of 3 independent experiments.