Elevated TAPP2 and syntrophin expression in the ZAP70⁺, IgH unmutated subset of B-CLL patients. Protein extracts of B-CLL (CLL) or normal human B cells (NB) were made using NP-40 lysis buffer, and 5 μg of protein per sample was run on SDS-PAGE gels. (A) TAPP2, ZAP70, or actin expression was detected by Western blot. Blots are representative of > 40 samples analyzed (each sample examined in at least 2 independent experiments). (B) Chemiluminescence signals for TAPP2, ZAP70, and actin blots were quantified using a Fluorchem instrument. Data are normalized to the actin signal for each sample. Graphs represent the normalized expression of 41 CLL and 15 normal B-cell samples, and CLL samples were divided into IgH unmutated/ZAP70⁺ and IgH mutated/ZAP70⁻ groups. P values were calculated using a Mann-Whitney test comparing the indicated groups. (C) Representative Western blot showing syntrophin expression in CLL and normal B cells. (D) Quantitative analysis of syntrophin expression in normal B cells, and unmutated/ZAP70⁺ and IgH mutated/ZAP70⁻ CLL groups.