De novo–synthesized, STAT6-dependent factors are necessary for Cdh1 transcription. (A) WT BALB/c thio-PEMs, either pretreated or not with 100nM JAK inhibitor I, 50nM wortmannin (PI3K inhibitor), or 6μM SB203580 (p38 MAPK inhibitor), were IL-4 steered for 6 or 24 hours. STAT6^−/− BALB/c thio-PEMs were IL-4 steered during 6 or 24 hours. (B) BALB/c thio-PEMs were pretreated with cycloheximide to block de novo protein synthesis, followed by 1 or 6 hours of IL-4 stimulation, and Cdh1 expression was determined. The fold induction of gene expression relative to the expression in the corresponding non–IL-4–treated thio-PEMs (n = 1) is shown. (C) WT BALB/c thio-PEMs, whether or not pretreated with 10μM GW9662 (PPAR-γ inhibitor) or 10μM L-165, 041 (PPAR-δ ligand), were IL-4 steered for 24 hours. Fold Cdh1 induction in IL-4–treated thio-PEM is set to 100%, and the effect of PPAR modifiers is shown relative to this level. WT or Gal3^−/− C57BL/6 thio-PEMs were IL-4–stimulated for 24 hours, and the fold induction in IL-4–treated Gal3^−/− thio-PEMs is shown relative to the induction level in WT thio-PEMs (100%). Values represent the mean ± SEM of 3 mice.*P < .05. **P < .01. ns indicates not significant.