Inactivation of Rent 1. (A) Using a siRNA against Rent 1, this gene was inactivated in Raji cells, a B-cell line. The bar diagram shows relative expression of Rent 1 RNA by real-time RT-PCR analysis in untreated cells, Raji cells transfected with scrambled siRNA, or with Rent 1 siRNA (relative to actin expression). (B) The same cDNA material was analyzed by PCR with primers in exons 10 and 12. An exon 11–skipped transcript is seen in the Rent 1–transfected Raji cells, as indicated by the lower ←.