E-cadherin expression in CLL specimens. Total E-cadherin expression was determined by real-time PCR analysis in 35 different CLL specimens from patients. The PCR primers and probe used for analysis detect the junction of exon 10-11. The bar diagram is a relative expression of E-cadherin in CLL specimens compared with normal peripheral blood B cells (n = 4) and adjusted to actin. On the x-axis, CLL specimens are grouped according to Rai clinical stage.