Obesity reduces lymphoid pool and increases myeloid progenitors. (A) BM cells from femurs of control and DIO mice (13 months of age; n = 6/group) were stained for lineage markers, CD117, Sca1, and CD127. A representative dot plot show gating strategy (on Lin^lo cells) and Flt3 expression to define MPP (Flt3⁺LSK) and CLPs (Lin⁻Sca1⁺CD117^loCD127⁺) with CD127 expression depicted as histogram. (B) Flow cytometry analyses of BM cells from 13-month-old control and DIO mice. Obesity increases the subsets of CMPs (Lin⁻CD127⁻CD117^hiSca1 CD34⁺CD16CD32⁻) and MEPs (Lin⁻CD127⁻CD117^hiSca1⁻CD34⁻CD16CD32⁻) and significantly reduced (P < .05) GMPs (Lin⁻CD127⁻CD117^hiSca1⁻CD34⁺CD16CD32⁺).