Mc4r-mediated adiposity induces premature restriction of TCR diversity. (A) The TCR spectratyping analysis of CD4 T cells from control and Mc4r-null mice is shown. The x-axis shows CDR3 lengths, and each line crossing on the y-axis of the landscape denotes perturbation for a specific CDR3 size (x-axis), whereas individual Vβ are shown on the z-axis. The mountain (in green, yellow, and orange) depicts perturbation in amplified peaks of CDR3 lengths of MC4R^−/− animals in comparison to WT mice (n = 5). (B) Representative Vβ results of CDR3 size spectratyping in WT and Mc4r-null mice.