American Society of Hematology

Figure 1

$Figure 1. Procedure for determining 2H enrichments in CD38+ and CD38− CLL cells and time point selection in relation to 2H availability in body water. (A) Deuterium enrichment measured in plasma of body water compartment of a representative case (CLL 875). CLL cells were flow-sorted at the 3 time points indicated by arrows. Vertical dashed line indicates time when 2H2O intake was ended. (B) After gating CD19+CD3− cells, CD5+ cells were flow-sorted based on their expression of CD38. 2H in genomic DNA was then measured by GC/MS. (C) The ratio of CD38+/CD38− fractions (f) of labeled cells (Table 2) was averaged. Bars represent SEs of the 3 time points studied. Significant differences in 2H incorporation between the CD38− and CD38+ B-CLL subpopulations were achieved early and maintained during labeling period, whereas they disappeared during washout.$

Procedure for determining ²H enrichments in CD38⁺ and CD38⁻ CLL cells and time point selection in relation to ²H availability in body water. (A) Deuterium enrichment measured in plasma of body water compartment of a representative case (CLL 875). CLL cells were flow-sorted at the 3 time points indicated by arrows. Vertical dashed line indicates time when ²H₂O intake was ended. (B) After gating CD19⁺CD3⁻ cells, CD5⁺ cells were flow-sorted based on their expression of CD38. ²H in genomic DNA was then measured by GC/MS. (C) The ratio of CD38⁺/CD38⁻ fractions (f) of labeled cells (Table 2) was averaged. Bars represent SEs of the 3 time points studied. Significant differences in ²H incorporation between the CD38⁻ and CD38⁺ B-CLL subpopulations were achieved early and maintained during labeling period, whereas they disappeared during washout.

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