HKH20 improves survival in a mouse model of S pyogenes sepsis. (A) Mice were injected subcutaneously in the neck with 2 × 107 CFU S pyogenes bacteria and treated with 100 μL HKH20 (200 μg; □) or 100 μL PBS (■) intraperitoneally 8 hours after infection (n = 4 or 5/group). Mortality was recorded for a period of 5 days. The experiment was repeated 4 times, and the results from a total of 17 animals per group are shown. (B) Mice were infected subcutaneously with 2 × 107 CFU S pyogenes bacteria and treated with 10 mg/kg clindamycin (▴) or 200 μg HKH20 and 10 mg/kg clindamycin (□) in a volume of 200 μL PBS. Treatment was intraperitoneal injection at 18, 42, 48, and 72 hours after infection (n = 5/group). Mortality was recorded for a period of 7 days. The experiment was repeated 3 times, and the results from a total of 15 animals per group are shown.
