BHQ880 inhibits IL-6 production and MM cell adhesion and modulates β-catenin and NF-κB pathways in BMSC from MM patients. BMSC were incubated for 24 hours with 1 μg/mL isotype control or BHQ880. Cells were harvested and subjected to cell lysis. (A) Cytoplasmic fraction was subjected to immunoblotting using anti-phospho β-catenin antibody and anti–β-catenin antibody. (B) Nuclear fraction was subjected to EMSA for NF-κB binding activity. Oct-1 DNA binding activity by EMSA was used as internal control. (C) Nuclear proteins (15 μg) were analyzed for NF-κB activity using the Transcription Factor ELISA kit, which measures DNA binding activity. Absorbance was obtained with a spectrophotometer at 450 nm and is presented as optical density (OD).