Sox7-enforced expression maintains the proliferation of early hematopoietic precursors. (A) CD41 and CD34 staining on cells derived from day 5 iSox7 EBs. (B) Unsorted, CD41⁺CD34⁻-, and CD41⁺CD34⁺-sorted cells from day 5 iSox7 EBs were tested in semisolid clonogenic replating assay for hematopoietic progenitors with or without doxycycline. (C) Cell-cycle status was assessed after 1 hour BrdU pulse on CD41⁺CD34⁻ cells culture for 4 days (D4) +dox or −dox. (D) Sorted CD41⁺CD34⁻ cells were cultured for 8 days + or − doxycycline. Total cell count was determined every other day (n = 5). All data are representative of at least 3 independent experiments. Error bars represent SEM.