Sox7 down-regulation leads to erythroid and myeloid differentiation. (A) GFP expression on pooled colonies harvested after 6 days in liquid hematopoietic conditions with doxycycline (red line) compared with GFP expression after doxycycline removal for 1 day (black line) and 2 days (green line). (B) Flow cytometry analysis of iSox7⁺ cells cultured in liquid hematopoietic conditions for the indicated time without doxycycline. (C) May-Grünwald Giemsa staining at days 6 and 10 of culture after doxycycline removal. (D) After May-Grünwald Giemsa staining, the percentage of erythroid and myeloid cell types was scored from individual iSox7⁺ colonies grown for 5 days without doxycycline. For each colony, 100 cells were counted. (E) Individual iSox7⁺ blastic colonies from clonogenic replating with doxycycline were plated in secondary clonogenic assay without doxycycline. All data are representative of at least 3 independent experiments.