Syk-deficient neutrophils have altered cytokine profiles after stimulation with bacteria. (A) WT (white bars) or syk^−/− (black bars) neutrophils were stimulated with rag1^−/− serum-opsonized S aureus or E coli (MOI = 5) for 16 hours. Culture supernatants or cell pellets lysed in low detergent lysis buffer were analyzed for TNF-α, MIP1α, MIP2, KC, IL-1β, IP-10, or MCP1 by multiplex bead array (Milliplex). Data are the mean of 2 independent experiments, each with an N = 2 or N = 3. (B) WT, syk^−/−, CD11b^−/−, or CD18^−/− neutrophils were stimulated with rag1^−/− serum-opsonized S aureus or E coli (MOI = 2) for 16 hours and assessed for (B) TNF-α secretion into the supernatant or (C) TNF-α synthesis and storage in cell lysates by plate-based ELISA, and represent at least 3 independent experiments. Data are presented as mean ± SD. *P < .05, **P < .01, # P < .001 compared with WT by 2-way ANOVA with Bonferroni posttests.