miRNAs 21 and 34a together further regulate MDDC differentiation. Monocytes were either untransfected or transfected with Cy3-labeled nontargeting negative anti-miR and anti–miR-21, anti–miR-34a, or both. DC-SIGN/CD14 expression was normalized to MFI ratios of untransfected MDDCs at day 5. Ratios were calculated using MFI of DC-SIGN/ MFI of CD14. P values were calculated using a paired t test comparison with the untransfected MDDCs: *P < .05; **P < .01; ***P < .001. Scatter plots show data from 4 independent donors. Monocytes were differentiated in the presence of GM-CSF and IL-4. Cells were stained and analyzed at day 5 of MDDC differentiation for DC-SIGN and CD14 cell surface expression. Live cell populations were further gated on the top 30% of positively transfected (Cy3⁺) cells for analysis.