WNT1 and JAG1 are target genes of miR-21, miR-34a, and let-7e. Luciferase reporter gene assays of vector constructs with (A) JAG1-3′-UTR (left of dotted line) and JAG1mut 34a-3′-UTR (right of dotted line) or (B) WNT1-3′-UTR (left side) and WNT1mut34a-3′-UTR (right side) in the absence or presence of the indicated miRNAs. The luciferase expression of each vector used was set to 1 and used for normalization of all the respective assays. The negative miRNA control is nontargeting, having no sequence homology to the human genome. Error bars represent SEM from 3 independent experiments. P values (above each bar) were calculated using a paired t test comparison of the condition with the no miRNA condition: *P < .05; **P < .01; ***P < .001.