Discordance between JAG1 and WNT1 mRNA and protein expression levels. Quantitative RT-PCR of (A) JAG1 and (B) WNT1 mRNA in day 0, 1, 3, and 5 differentiated MDDCs. One representative donor from 3 is shown. Error bars represent SD from 3 replicates. (C) Immunoprecipitation-Western blot of Jagged-1 protein (∼ 180 kDa) in lysate (350 μg protein per sample). (D) Top: Western blot detection of Wnt-1 protein (∼ 41 kDa) in lysate (33 μg protein per lane) from day 0, 1, 3, and 5 differentiated MDDCs. Bottom: Western blot detection of actin loading control.